Administrative Guideline - 8453 Routine Procedures for Sanitation and Hygiene When Handling Body Fluids


To ensure that body fluids involving blood, vomitus, urine, feces, semen, saliva, and nasal discharges are handled properly.


All school staff should be alerted to dangers of infections from body fluids. School nurses, custodians, and teachers should be particularly alert to the proper techniques in handling and disposal of materials.


Disposal Bags
Dust Pans
Paper Towels
Disposable Gloves
Disinfectants - should be one of the following classes:

A.     phenolic germicidal detergent in a one (1) percent aqueous solution (e.g. Lysol*)

B.     sodium hypochlorite solution (household bleach), 1 part bleach to 10 parts water (e.g. 1-1/2 cups bleach to one (1) gallon of water. Needs to be prepared each time used.)

C.     quaternary ammonium germicidal detergent in 2% aqueous solution (e.g., Tri-quat*, Mytar*, or Sage*)

D.     iodophor germicidal detergent with 500ppm available iodine (e.g., Wescodyne*)

E.     sanitary absorbing agent (Chlora Sorb*, X-O Oder Away*)



     1. Wear disposable gloves before making contact with body fluids during care, treatment, and all cleaning procedures.

     2. Dispose of gloves after each use.

     3. Wash hands after handling fluids and contaminated articles, whether or not gloves are worn.

     4. Discard disposable items including tampons, used bandages, and dressings in plastic-lined trash container with lid. Close bags and dispose of daily.

     5. Do not reuse plastic bags.

     6. Use disposable items to handle body fluids whenever possible.

     7. Use paper towels to pick up and dispose of any solid waste materials such as vomitus or feces.


     1. Use soap and warm running water. Soap suspends easily removable soil and micro-organisms allowing them to be washed off.

     2. Rub hands together for approximately 10 seconds to work up a lather.

     3. Scrub between fingers, knuckles, backs of hands, and nails.

     4. Rinse hands under warm running water. Running water is necessary to carry away debris and dirt.

     5. Use paper towels to thoroughly dry hands.

     6. Dispose of paper towels.


     1. For tables, desks, etc.:

          a. Use Lysol*, or household bleach solution of one (1) part bleach to ten (10) parts water, mixed fresh.

          b. Rinse with water if so directed on disinfectant.

          c. Allow to air dry.

          d. When bleach solution is used, handle carefully.

               1) Gloves should be worn since the solution is irritating to skin.

               2) Avoid applying on metal since it will corrode most metals.

     2. For floors:

          a. One of the most readily available and effective disinfectants is the bleach solution (1-1/2 cups bleach to one (1) gallon water).

          b. Use the two (2) bucket system--one bucket to wash the soiled surface and one (1) bucket to rinse as follows:

               1) In bucket #1, dip, wring, mop up vomitus, blood.

               2) Dip, wring, and mop once more.

               3) Dip, wring out mop in bucket #1.

               4) Put mop into bucket #2 (rinse bucket) that has clean disinfectant (such as Lysol*, bleach solution).

               5) Mop or rinse area.

               6) Return mop to bucket #1 to wring out. This keeps the rinse bucket clean for second spill in the area.

               7) After all spills are cleaned up, proceed with "c.".

          c. Soak mop in the disinfectant after use.

          d. Disposable cleaning equipment and water should be placed in a toilet or plastic bag as appropriate.

          e. Rinse non-disposable cleaning equipment (dustpans, buckets) in disinfectant.

          f. Dispose disinfectant solution down a drain pipe.

          g. Remove gloves, if worn, and discard in appropriate receptacle.

          h. Wash hands as described above under HANDWASHING.

D.     FOR NONWASHABLE SURFACES (rugs, upholstery)

     1. Apply sanitary absorbing agent, let dry, vacuum.

     2. If necessary, use broom and dustpan to remove solid materials.

     3. Apply rug or upholstery shampoo as directed. Revacuum according to directions on shampoo.

     4. If using a water extraction cleaner, follow the directions on the label.

     5. Clean dustpan and broom, if used. Rinse in disinfectant solution.

     6. Air dry.

     7. Wash hands as described above under HANDWASHING.

E.     FOR SOILED WASHABLE MATERIALS (clothing, towels, etc.)

     1. Rinse item under running water using gloved hands if appropriate.

     2. Place item in plastic bag and seal until item is washed. Plastic bags containing soiled, washable material must be clearly identified if outside laundry service is used.

     3. Wash hands as described above under HANDWASHING.

     4. Wipe sink with paper towels, dispose of towels.

     5. Wash soiled items separately, washing and drying as usual.

     6. If material is bleachable, add 1/2 cup bleach to the wash cycle. Otherwise, add 1/2 cup non-chlorine beach (Clorox II*, Borateem*) to the wash cycle.

     7. Dispose of plastic bag.

     8. Wash hands as described above under HANDWASHING after handling soiled items.







  • cuts/abrasions
  • nosebleeds
  • menses
  • contaminated needle
Hepatitis B virus

AIDS virus

Bloodstream inoculation
through cuts and abrasions on hands

Direct bloodstream inoculation
  • incontinence
Salmonella bacteria

Shigella bacteria


Hepatitis A virus
Oral inoculation from contaminated hands
  • incontinence
Cytomegalovirus Bloodstream, oral and mucus membrane inoculation from hands
 ***Respiratory Secretions
  • saliva
  • nasal discharge
Mononucleosis virus

Common cold virus

Influenza virus
Oral inoculation from contaminated hands
 ***Vomitus Gastrointestinal viruses

e.g. (Norwalk agent Rotavirus)
Oral inoculation from contaminated hands
Semen Hepatitis B virus

AIDS virus

Sexual contact

* Brand names are used as examples and are not endorsements of products.
** This is not an all inclusive list of organisms of concern for transmission in the school setting.
*** Possible transmission of AIDS is currently thought to be of little concern from these sources.